Composite
Part:BBa_K3257109
Designed by: Yi Qiao Group: iGEM19_Fudan-TSI (2019-10-15)
gag-Q-pol with ptetR Promoter
This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and ptetR. Under different regulation by different promoters, the expression level varies accordingly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 259
Illegal BglII site found at 1371
Illegal BglII site found at 2677
Illegal BglII site found at 3909
Illegal BamHI site found at 2694
Illegal BamHI site found at 3000
Illegal XhoI site found at 1025 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1910
Illegal AgeI site found at 3481 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |